When and where does DNA replication take place?
This process takes place during the interphase (see link mitosis), in which all the necessary preparations for the forthcoming cell division take place. Replication is regulated by some enzymes (proteins). Before DNA can be duplicated, the DNA double strand must be separated.
Why is there replication?
This means that the existing cells have to divide so that one cell becomes two. However, each of these cells must receive the entire genetic information, the DNA, otherwise it is not viable. For this, the DNA must be doubled before the actual cell division. This process is called DNA replication.
Why Discontinuous Replication?
The DNA polymerase runs discontinuously on the other strand, since it can only link there away from the replication fork. The resulting gaps between the completed Okazaki pieces are joined into a continuous strand by a specific enzyme, DNA ligase.
Which enzymes are involved in the duplication of DNA?
Both strands serve as a template for the amplification of the DNA. The enzymes helicase, primase, DNA polymerase I & III and DNA ligase are involved.
Where are Okazaki fragments formed?
The sought-after Okazaki fragments arise during replication. In this process, the DNA double helix is separated and new complementary strands are formed on both antiparallel single strands (semiconservative replication).
Which enzyme is responsible for transcription?
During transcription (lat. transscribere=to write over/rewrite) the information required for protein production is transcribed from the DNA into an mRNA (messenger). This is done by a specially responsible enzyme, the RNA polymerase.
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